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M94B0801.TXT
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1994-11-11
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Document 0801
DOCN M94B0801
TI Inhibition of herpes simplex virus 1 reproduction with hydrophobized
antisense oligonucleotides.
DT 9412
AU Vinogradov SV; Suzdaltseva Y; Alakhov VYu; Kabanov AV; Moscow Institute
of Biotechnology Inc., Russia.
SO Biochem Biophys Res Commun. 1994 Sep 15;203(2):959-66. Unique Identifier
: AIDSLINE MED/94380082
AB Antisense oligonucleotides were modified by their ends with hydrophobic
substituents which permitted enhancing their activity. The effect of
hydrophobized oligonucleotides on reproduction of Herpes Simplex Virus
type 1 in Vero cells was studied. Two types of oligonucleotides were
used: a 12-mer complementary to the splicing site 983-994 of early
mRNA-5 of the virus and 19-mer complementary to the site of mRNA that
encodes the virus DNA-polymerase. These oligonucleotides were modified
by 5'-ends with n-undecyl or cholesteryl moieties and by 3'-ends with
acridine or 1,7-heptanediol. In comparison with the unmodified
oligonucleotides the hydrophobized ones were significantly more active
and inhibited HSV-1 infection at micromolar concentrations.
DE Acridines/CHEMISTRY Animal Base Sequence Cholesterol/CHEMISTRY DNA
Polymerases/GENETICS Glycols/CHEMISTRY HIV-1/DRUG EFFECTS/*GROWTH &
DEVELOPMENT Molecular Sequence Data Oligonucleotides,
Antisense/CHEMISTRY/*PHARMACOLOGY RNA Splicing RNA, Messenger RNA,
Viral Vero Cells JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).